: Traditional selective breeding is not considered genetic engineering. Core Technologies and Methods
: Herbert Boyer and Stanley Cohen achieved the first artificial modification in 1973.
: Includes gene targeting, nuclear transplantation, and viral insertion .
: Using genes from the same or closely related species.
: The most widely applied method for targeting specific sequences .
: Used to insert therapeutic genes into host genomes. Major Applications
: Tools that create double-strand breaks to allow DNA editing.
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