C18zip Apr 2026

Aspirate and dispense your peptide sample through the ZipTip.

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The sample is now ready for (MALDI-TOF or LC-MS/MS). C18zip

Repeat this cycle to maximize binding to the C18 media. Note: Slow, consistent pipetting is key for high recovery. 5. Washing (Desalting) Aspirate the Wash Solution (0.1% TFA). Dispense to waste. Repeat 3 times to remove salts, buffers, and detergents. 6. Elution Aspirate the Elution Solution (e.g., 60% ACN/0.1% TFA).

50–70% ACN in 0.1% TFA (depending on peptide hydrophobicity). 2. Wetting the Tip Set your pipette to 10 µL. Aspirate the Wetting Solution (100% ACN) into the tip. Dispense to waste. Repeat 2 times . This activates the C18 resin. 3. Equilibration Aspirate the Equilibration Solution (0.1% TFA). Dispense to waste. Aspirate and dispense your peptide sample through the ZipTip

Below is the standard protocol for peptide desalting and concentration using C18 ZipTips. 🧪 C18 ZipTip Standard Protocol 1. Preparation & Materials

Dispense the liquid into a clean, sterile microcentrifuge tube. Repeat this cycle to maximize binding to the C18 media

Repeat . This prepares the resin to bind the peptides. 4. Sample Binding