This volume collects the following US issues, originally published in 2010: Web of Spider-Man (Vol. 2) #4–5 Key Storyline: "The Gauntlet" (El Desafío)
: Dan Slott, Fred Van Lente, Mark Waid, and Tom Peyer.
: In addition to Mysterio, the volume features confrontations with Mister Negative , Morbius , and a new iteration of the Vulture .